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Original Research
6.
Study on the incidence of Salmonella enteritidis in
poultry and meat samples by cultural and PCR methods - Putturu Ramya, Thirtham Madhavarao, Lakkineni
Venkateswara Rao
Vet World. 2012; 5(9): 541-545
doi:
10.5455/vetworld.2012.541-545
Abstract
Aim:
To study the incidence of S.enteritidis in poultry
and meat samples by cultural and PCR methods.
Materials and Methods: A total of 130 samples
(25 each of chicken, mutton, poultry faeces, cloacal
samples and 10 each of liver, spleen and kidney)
collected from different sources were subjected to
cultural and PCR methods for the presence of
Salmonella and Salmonella enteritidis. Primers for
invA and sefA gene were used for Salmonella and
S.enteritidis respectively.
Results: Out of
130 samples, 87 were positive for Salmonella spp.
i.e. chicken-16(64%), mutton-12(48%),
faeces-23(92%), cloacal swabs-23(92%), liver-5(50%),
spleen and kidney samples-4(40%) each by PCR
methods, whereas 77 were positive by cultural method
i.e. chicken-14(56%), mutton-10(40%),
faeces-22(88%), cloacal swabs-21(84%), liver-4(40%),
spleen and kidney-3(30% each). Out of 87 positive
for Salmonella by PCR method, 59(chicken-12,
mutton-7, faeces-17, cloacal swabs-15, liver-3,
spleen-2, kidney-3) were positive for S.enteritidis.
High incidence of S.enteritidis (68%) in all the
above samples are indicative of unhygienic
conditions in poultry farms. Selective enrichment
with Rappaport-Vassilidias (RV) broths and
Tetrathionate (TT) broths were superior over
Selenite-F (SF) and Selenite cysteine (SC) broths.
Conclusions: High incidence of S.enteritidis
was seen in most of poultry samples like chicken,
kidney, liver and it's faeces than mutton, which was
indicative of contamination of S.enteritidis is more
prevalent in poultry farms. Keywords:
Cultural, Incidence, PCR, Salmonella, Salmonella
enteritidis.
